Evaluating Nanofiltration Effect on Wine 87Sr/86Sr Isotopic Ratio and the Robustness of this Geographical Fingerprint

Cíntia Moreira, Maria de Pinho, António Curvelo-Garcia, Bruno de Sousa, Jorge Ricardo-da-Silva, Sofia Catarino


When aiming to use the strontium isotopic ratio 87Sr/86Sr for the traceability and authentication of wine, it is crucial to understand the impacts of anthropogenic factors and technological processes on this parameter.  A study was developed to evaluate the effect of nanofiltration (NF), a membrane process with several applications in oenology, on the isotopic ratio 87Sr/86Sr of wine, testing its robustness as geographical tracer.  NF assays, for dealcoholisation, were carried out at laboratory scale with model solutions and white and red wines from Portuguese DO. High rejections to strontium (97%) and tartaric acid (> 70%) and low rejection to ethanol (< 10%) at 15 bar were observed in model solutions. Wines and NF-processed wines (17 bar; 2.1 L/min) were evaluated for physicochemical composition (including phenolic composition, chromatic characteristics and polysaccharides), multi-elemental composition and 87Sr/86Sr ratio by Q-ICPMS.  The preferential membrane permeation of ethanol was associated with a slight increase in the quality parameters of the wine (anthocyanins and polysaccharides). High rejections to mineral elements, including heavy metals, were observed. Rejection to Sr was mostly higher than 95%. No significant differences in the 87Sr/86Sr of wines and corresponding permeate fractions were observed, suggesting that NF does not preclude the use of this parameter for wine traceability purposes.


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Almeida, C. M. R., & Vasconcelos, M. T. S. D., 2001. ICP-MS determination of strontium isotope ratio in wine in order to be used as a fingerprint of its regional origin. J. Anal. At. Spectrom. 16 (6), 607–611.

Almeida, C. M. R. & Vasconcelos, M. T. S. D., 2004. Does the winemaking process influence the wine 87Sr/ 86Sr? A case study. Food Chem. 85, 7–12.

Barbaste, M., Robinson, K., Guilfoyle, S., Medina, B. & Lobinski, R., 2002. Precise determination of the strontium isotope ratios in wine by inductively coupled plasma sector field multicollector mass spectrometry (ICP-SF-MC-MS). J. Anal. Atom. Spectrom. 17 (2), 135–137.

Capo, R. C., Stewart, B. W. & Chadwick, O. A., 1998. Strontium isotopes as tracers of ecosystem processes: theory and methods. Geoderma 82 (1-3), 197–225.

Catarino, M. & Mendes, A., 2011. Dealcoholizing wine by membrane separation processes. Innov. Food Sci. Emerg. 12 (3), 330–337.

Catarino, S., Curvelo-Garcia, A. S. & Bruno de Sousa, R., 2006. Measurements of contaminant elements of wines by inductively coupled plasma-mass spectrometry: A comparison of two calibration approaches. Talanta 70 (5), 1073–1080.

Catarino, S., Madeira, M., Monteiro, F., Rocha, F., Curvelo-Garcia, A. S. & Bruno de Sousa, R., 2008a. Effect of bentonite characteristics on the elemental composition of wine. J. Agric. Food Chem. 56 (1), 158–165.

Catarino, S., Curvelo-Garcia, A. S. & Bruno de Sousa, R., 2008b. Contaminant elements in wines: a review. Ciência Téc. Vitiv. 23 (1), 3–19.

Catarino, S., Trancoso, I. M., Bruno de Sousa, R. & Curvelo-Garcia, A. S., 2010. Grape must mineralization by high pressure microwave digestion for trace element analysis: development of a procedure. Ciência Téc. Vitiv. 25 (2), 87–93.

Catarino S., Madeira M., Monteiro M., Caldeira I., Rosa T., Martins P., Bruno de Sousa R. & Curvelo-Garcia A.S., 2014. Multi-elemental analysis throughout soil-wine system as a generator of information on geographic origin. In: Proc. 37th World Congress of Vine and Wine, November 2014, Mendoza, Argentina.

Catarino, S., Bruno de Sousa, R. & Curvelo-Garcia, A. S., 2015. Origem geográfica. In: Curvelo-Garcia, A.S. & Barros, P. (eds). Química enológica – métodos analíticos. Avanços recentes no controlo da qualidade de vinhos e de outros produtos vitivinícolas. Publindústria, Porto. pp. 513-538.

de Pinho, M. N., Matsuura, T., Nguyen, T. D. & Sourirajan, S., 1988. Reverse osmosis separation of glucose-ethanol-water system by cellulose acetate membranes. Chem. Eng. Commun. 64 (1), 113–123.

Durante, C., Baschieri, C., Bertacchini, L., Bertelli, D., Cocchi, M., Marchetti, A., Manzini, D., Papotti, G. & Sighinolfi, S., 2015. An analytical approach to Sr isotope ratio determination in Lambrusco wines for geographical traceability purposes. Food Chem. 173, 557–563.

Fernandes, J. R., Pereira, L., Jorge, P., Moreira, L., Gonçalves, H., Coelho, L., Alexandre, D., Eiras-Dias, J., Brazão, J., Clímaco, P., Baleiras-Couto, M., Catarino, S., Graça, A. & Martins-Lopes, P., 2015. Wine fingerprinting using a bio-geochemical approach. BIO Web of Conferences, 5, 02021.

Gonçalves, F. & de Pinho, M., 2003. Integrated nanofiltration process to reduce the alcohol content of alcoholic beverages, Patente de Invenção Nacional No102976.

Gonçalves, F., Ribeiro, R., Neves, L., Lemperle, T., Lança, M., Ricardo da Silva, J. & Laureano, O., 2013. Alcohol reduction in wine by nanofiltration. Some comparisons with reverse osmosis technique. In: Teissedre P.-L. (Coord.). Proc. 1st International Symposium of Oenoviti International Network. September 2013, Bordeaux, France, pp. 64–67.

Horn, P., Schaaf, P., Holbach, B., Hölzl, S. & Eschnauer, H., 1993. 87Sr/86Sr from rock and soil into vine and wine. Z. Lebensm. Unters. Forsch. 196 (5), 407–409.

Horn, P., Hölzl, S., Todt, W. & Matthies, D., 1997. Isotope abundance ratios of Sr in wine provenance determinations, in a tree-root activity study, and of Pb in a pollution study on tree-rings. Isotopes Environ. Health Stud. 33 (1-2), 31–42.

Kunst, B. & Sourirajan, S., 1970. Performance of some improved porous cellulose acetate membranes for low pressure reverse osmosis desalination. Desalination 8 (1), 139–152.

Marchionni, S., Braschi, E., Tommasini, S., Bollati, A., Cifelli, F., Mulinacci, N., Mattei, M. & Conticelli, S., 2013. High-precision 87Sr/86Sr analyses in wines and their use as a geological fingerprint for tracing geographic provenance. J. Agric. Food Chem. 61 (28), 6822–6831.

Marchionni, S., Buccianti, A., Bollati, A., Braschi, E., Cifelli, F., Molin, P., Parotto, M., Mattei, M., Tommasini, S. & Conticelli, S., 2016. Conservation of 87Sr/86Sr isotopic ratios during the winemaking processes of “Red” wines to validate their use as geographic tracer. Food Chem. 190, 777–785.

Martins, P., Madeira, M., Monteiro, F., Bruno de Sousa, R., Curvelo-Garcia, A. S. & Catarino, S., 2014. 87Sr/86Sr ratio in vineyards soils from portuguese denominations of origin and its potencial for origin authentication. J. Int. Sci. Vigne Vin 48, 21–29.

Massot, A., Mietton-Peuchot, M., Peuchot, C. & Milisic, V., 2008. Nanofiltration and reverse osmosis in winemaking. Desalination 231, 283–289.

Mercurio, M., Grilli, E., Odierna, P., Morra, V., Prohaska, T., Coppola, E., Grifa, C., Buondonno, A. & Langella, A., 2014. A “Geo-Pedo-Fingerprint” (GPF) as a tracer to detect univocal parent material-to-wine production chain in high quality vineyard districts, Campi Flegrei (Southern Italy). Geoderma 230-231, 67–78.

Mohammad, A. W., Teow, Y. H., Ang, W. L., Chung, Y. T., Oatley-Radcliffe, D. L. & Hilal, N., 2014. Nanofiltration membranes review: Recent advances and future prospects. Desalination 356, 226–254.

OIV, 2014. Compendium of International Methods of Wine and Must Analysis I/II. Paris, France: Organisation Internationale de la Vigne et du Vin, Paris, France.

OIV, 2015. International Code of Oenological Practices. Organisation Internationale de la Vigne et du Vin, Paris, France.

Rosner, M., 2010. Geochemical and instrumental fundamentals for accurate and precise strontium isotope data of food samples: Comment on “Determination of the strontium isotope ratio by ICP-MS ginseng as a tracer of regional origin” (Choi et al., 2008). Food Chem. 121 (3), 918–921.

Salgado, C., Palacio, L., Carmona, F. J., Hernández, A. & Prádanos, P., 2013. Influence of low and high molecular weight compounds on the permeate flux decline in nanofiltration of red grape must. Desalination 315, 124–134.

Somers, T. C. & Evans, M. E., 1977. Spectral Evaluation of Young Red Wines: Anthocyanin Equilibria, Total Phenolics, Free and Molecular SO2, “Chemical Age. J. Sci. Food Agr. 28, 279 – 287.

Szöke, S., Pátzay, G. & Weiser, L., 2005. Cobalt(III) EDTA complex removal from aqueous alkaline borate solutions by nanofiltration. Desalination 175, 179–185.

Victor, V., Ross, S., Karine, P., André, P., Jean-François, H. & David, W., 2015. Strontium isotope characterization of wines from the Quebec (Canada) Terroir. Procedia Earth and Planetary Science 13 , 252–255.

Vorster, C., Greeff, L. & Coetzee P.P., 2010. The determination of 11B/10B and 87Sr/86Sr isotope ratios by quadrupole-based ICP-MS for the fingerprinting of South African Wine. S. Afr. J. Chem. 63, 207-214.

DOI: http://dx.doi.org/10.21548/38-1-942


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