Effect of Training Systems on Accumulation of Flavan-3-ols in Cabernet Sauvignon Grape Seeds at the North Foot of Mt. Tianshan

Y. Liu, J. Yan, Q. Li, J. Wang, Y. Shi


development of Cabernet Sauvignon grapes during two consecutive seasons (2011 to 2012) in the northwest of China was evaluated. The applied training systems include the modified vertical shoot positioned
(M-VSP); the fan training system with two trunks (F-TT); and the fan training system with multiple trunks (F-MT). The results show that the pattern of flavan-3-ol accumulation was similar for the three training systems in both vintages, with flavan-3-ols being the highest around véraison, before declining as harvest approaches. Compared to the 2011 vintage, the dry vintage of 2012 had a greater accumulation of total flavan-3-ols and individual flavan-3-ol monomers, including (+)-catechin (C), (-)-epicatechin (EC) and (-)-epicatechin-3-O-gallate (ECG). With regard to the effect of training systems, M-VSP contributed to a significantly higher content of total flavan-3-ols, while the percentage of ECG was lower for M-VSP.  As for the three flavan-3-ol units (terminal subunits, extension subunits and monomers), the 2012 vintage was more likely to accumulate extension subunits and monomers, and the effect of training system on
these three flavan-3-ols units varied between the different vintages. Meanwhile, the proportion of ECG in terminal subunits and of C in extension subunits was higher in 2012, whereas the training system of F-TT
had a higher percentage of ECG in extension subunits in both vintages. In addition, the effect of vintage on the composition of flavan-3-ols in harvested grape seeds was more obvious than that of the training system.

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Alcalde-Eon, C., Escribano-Bailón, M.T., Santos-Buelga, C. & Rivas-Gonzalo, J.C. 2006. Changes in the detailed pigment composition of red wine during maturity and ageing: A comprehensive study. Anal. Chim. Acta. 563(1), 238-254.

Aron, P.M. & Kennedy, J.A. 2008. Flavan-3-ols: Nature, occurrence and biological activity. Mol. Nutr. Food Res. 52(1), 79-104.

Bavougian, C.M., Read, P.E., Schlegel, V.L. & Hanford, K.J. 2013. Canopy light effects in multiple training systems on yield, soluble solids, acidity, phenol and flavonoid concentration of ‘Frontenac’ grapes. HortTechnology 23(1), 86-92.

Bordiga, M., Travaglia, F., Locatelli, M., Coïsson, J.D. & Arlorio, M. 2011. Characterisation of polymeric skin and seed proanthocyanidins during ripening in six Vitis vinifera L. cv. Food Chem. 127(1), 180-187.

Cheng, G., He, Y.N., Yue, T.X., Wang, J. & Zhang, Z.W. 2014. Effects of climatic conditions and soil properties on Cabernet Sauvignon berry growth and anthocyanin profiles. Molecules 19(9), 13683-13703.

De Freitas, V.A.P. & Glories, Y. 1999. Concentration and compositional changes of procyanidins in grape seeds and skin of white Vitis vinifera varieties. J. Sci. Food Agr. 79(12), 1601-1606.

Downey, M.O., Harvey, J.S. & Robinson, S.P. 2003. Analysis of tannins in seeds and skins of Shiraz grapes throughout berry development. Aust. J. Grape Wine R. 9(1), 15-27.

Downey, M.O., Harvey, J.S. & Robinson, S.P. 2004. The effect of bunch shading on berry development and flavonoid accumulation in Shiraz grapes. Aust. J. Grape Wine R. 10(1), 55-73.

Fragasso, M., Antonacci, D., Pati, S., Tufariello, M., Baiano, A., Forleo, L.R., Caputo, A.R. & Notte, E.L. 2012. Influence of training system on vand sensory profiles of Primitivo grapes and wines. Am. J. Enol. Viticult. 63(4), 477-486.

Fulcrand, H., Dueñas, M., Salas, E. & Cheynier, V. 2006. Phenolic reactions during winemaking and aging. Am. J. Enol. Viticult. 57(3), 289-297.

Geny, L., Saucier, C., Bracco, S., Daviaud, F. & Glories, Y. 2003. Composition and cellular localization of tannins in grape seeds during maturation. J. Agr. Food Chem. 51(27), 8051-8054.

Giovanelli, G. & Brenna, O.V. 2007. Evolution of some phenolic components, carotenoids and chlorophylls during ripening of three Italian grape varieties. Eur. Food Res. Technol. 225(1), 145-150.

Gladstone, E.A. & Dokoozlian, N.K. 2003. Influence of leaf area density and trellis/training system on the light microclimate within grapevine canopies. Vitis 42(3), 123-131.

González-Neves, G., Gil, G. & Ferrer, M. 2002. Effect of different vineyard treatments on the phenolic contents in Tannat (Vitis vinifera L.) grapes and their respective wines. Food Sci. Technol. Int. 8(5), 315-321.

Hanlin, R., Hrmova, M., Harbertson, J. & Downey, M. 2010. Review: Condensed tannin and grape cell wall interactions and their impact on tannin extractability into wine. Aust. J. Grape Wine R. 16(1), 173-188.

Hanlin, R.L. & Downey, M.O. 2009. Condensed tannin accumulation and composition in skin of Shiraz and Cabernet Sauvignon grapes during berry development. Am. J. Enol. Viticult. 60(1), 13-23.

Hanlin, R.L., Kelm, M.A., Wilkinson, K.L. & Downey, M.O. 2011. Detailed characterization of proanthocyanidins in skin, seeds, and wine of Shiraz and Cabernet Sauvignon wine grapes (Vitis vinifera). J. Agr. Food Chem. 59(24), 13265-13276.

Haslam, E. 1998. Practical polyphenolics: from structure to molecular recognition and physiological action. Cambridge University Press, London.

Jones, H.G. 2013 (4th ed). Plants and microclimate: A quantitative approach to environmental plant physiology. Cambridge university press, London.

Katalinić, V. & Maleš, P. 1997. Compositional changes in grape polyphenols throughout maturation. J. Wine R. 8(3), 169-177.

Kemp, B.S., Harrison, R. & Creasy, G.L. 2011. Effect of mechanical leaf removal and its timing on flavan-3-ol composition and concentrations in Vitis vinifera L. cv. Pinot Noir wine. Aust. J. Grape Wine R. 17(2), 270-279.

Kennedy, J.A. & Jones, G.P. 2001. Analysis of proanthocyanidin cleavage products following acid-catalysis in the presence of excess phloroglucinol. J. Agr. Food Chem. 49(4), 1740-1746.

Kennedy, J.A., Matthews, M.A. & Waterhouse, A.L. 2000a. Changes in grape seed polyphenols during fruit ripening. Phytochemistry 55(1), 77-85.

Kennedy, J.A., Troup, G.J., Pilbrow, J.R., Hutton, D.R., Hewitt, D., Hunter, C.R., Ristic, R., Iland, P.G. & Jones, G.P. 2000b. Development of seed polyphenols in berries from Vitis vinifera L. cv. Shiraz. Aust. J. Grape Wine R. 6(3), 244-254.

Koyama, K., Ikeda, H., Poudel, P.R. & Goto-Yamamoto, N. 2012. Light quality affects flavonoid biosynthesis in young berries of Cabernet Sauvignon grape. Phytochemistry 78, 54-64.

Liang, N.N., He, F., Pan, Q.H., Wang, J., Reeves, M.J. & Duan, C.Q. 2014. Optimization of sample preparation and phloroglucinol analysis of Marselan grape skin proanthocyanidins using HPLC-DAD-ESI-MS/MS. S. Afr. J. Enol. Vitic. 33(1), 122-131.

Maury, C., Sarni-Manchado, P. & Lefebvre, S. 2001. Influence of fining with different molecular weight gelatins on proanthocyanidin composition and perception of wines. Am. J. Enol. Viticult. 52(2), 140-145.

Morrison, J.C. & Noble, A.C. 1990. The effects of leaf and cluster shading on the composition of Cabernet Sauvignon grapes and on fruit and wine sensory properties. Am. J. Enol. Viticult. 41(3), 193-200.

Liu, M.Y., Chi, M., Tang, Y.H., Song, C.Z., Xi, Z.M. & Zhang, Z.W. 2015. Effect of three training systems on grapes in a wet region of China: Yield, incidence of disease and anthocyanin compositions of Vitis vinifera cv. Cabernet Sauvignon. Molecules 20(10), 18967-18987.

Ough, C. & Nagaoka, R. 1984. Effect of cluster thinning and vineyard yields on grape and wine composition and wine quality of Cabernet Sauvignon. Am. J. Enol. Viticult. 35(1), 30-34.

Pérez-Lamela, C., García-Falcón, M.S., Simal-Gándara, J. & Orriols-Fernández, I. 2007. Influence of grape variety, vine system and enological treatments on the colour stability of young red wines. Food Chem. 101(2), 601-606.

Peleg, H., Gacon, K., Schlich, P. & Noble, A.C. 1999. Bitterness and astringency of flavan-3-ol monomers, dimers and trimers. J. Sci. Food Agr. 79(8), 1123-1128.

Prieur, C., Rigaud, J., Cheynier, V. & Moutounet, M. 1994. Oligomeric and polymeric procyanidins from grape seeds. Phytochemistry 36(3), 781-784.

Renaud, S.d. & de Lorgeril, M. 1992. Wine, alcohol, platelets, and the French paradox for coronary heart disease. The Lancet 339(8808), 1523-1526.

Reynolds, A.G. & Heuvel, J.E.V. 2009. Influence of grapevine training systems on vine growth and fruit composition: A review. Am. J. Enol. Viticult. 60(3), 251-268.

Riou, V., Vernhet, A., Doco, T. & Moutounet, M. 2002. Aggregation of grape seed tannins in model wine—effect of wine polysaccharides. Food Hydrocolloids 16(1), 17-23.

Ristic, R. & Iland, P.G. 2005. Relationships between seed and berry development of Vitis vinifera L. cv Shiraz developmental changes in seed morphology and phenolic composition. Aust. J. Grape Wine R. 11(1), 43-58.

Romeyer, F.M., Macheix, J.J. & Sapis, J.C. 1985. Changes and importance of oligomeric procyanidins during maturation of grape seeds. Phytochemistry 25(1), 219-221.

Sabbatini, P., Wierba, K., Clearwater, L. & Howell, G.S. 2015. Impact of training system and pruning severity on yield, fruit composition, and vegetative growth of ‘Niagara’ grapevines in Michigan. Int. J. Fruit Sci. 15(3), 237-250.

Santos-Buelga, C., Francia-Aricha, E. & Escribano-Bailón, M. 1995. Comparative flavan-3-ol composition of seeds from different grape varieties. Food Chem. 53(2), 197-201.

Segade, S.R., Vázquez, E.S., Rodríguez, E.I.V. & Martínez, J.F.R. 2009. Influence of training system on chromatic characteristics and phenolic composition in red wines. Eur. Food Res. Technol. 229(5), 763-770.

Vidal, S., Francis, L., Guyot, S., Marnet, N., Kwiatkowski, M., Gawel, R., Cheynier, V. & Waters, E.J. 2003. The mouth-feel properties of grape and apple proanthocyanidins in a wine-like medium. J. Sci. Food Agri. 83(6), 564-573.

Sun, B., Pinto, T., Leandro, M., Ricardo-Da-Silva, J. & Spranger, M. 1999. Transfer of catechins and proanthocyanidins from solid parts of the grape cluster into wine. Am. J. Enol. Viticult. 50(2), 179-184.

Xing, R.R., He, F., Xiao, H.L., Duan, C.Q. & Pan, Q.H. 2015. Accumulation pattern of flavonoids in Cabernet Sauvignon grapes grown in a low-latitude and high-altitude region. S. Afr. J. Enol. Vitic. 36(1), 32-43.

Xu, X.Q., Cheng, G., Duan, L.L., Jiang, R., Pan, Q.H., Duan, C.Q. & Wang, J. 2015. Effect of training systems on fatty acids and their derived volatiles in Cabernet Sauvignon grapes and wines of the north foot of Mt. Tianshan. Food Chem. 181, 198-206.

Zoecklein, B.W., Wolf, T.K., Pelanne, L., Miller, M.K., Birkenmaier, S.S. 2008. Effect of Vertical Shoot-Positioned, Smart-Dyson, and Geneva Double-Curtain training systems on Viognier grape and wine composition. Am. J. Enol. Vitic. 59, 11-21.

DOI: http://dx.doi.org/10.21548/39-1-1579


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